EYE DROP PRODUCT DEVELOPMENT FROM BUTTERFLY PEA EXTRACT

Abstract

Eye infections are prevalent among individuals and commonly treated with commercially available chemically synthesized eye drops. However, there is a growing interest in herbal medicine, with approximately 80% of the global population utilizing it. In line with this, the present study aims to develop herbal-based eye drops using butterfly pea as the main ingredient. Butterfly pea is recognized for its abundant anthocyanin content, which possesses potent therapeutic properties against eye infections. Ultrasound-Assisted Extraction, autoclave sterilization, disk diffusion test, and macrodilution were employed as key methodologies in this investigation. Results revealed that the double-flower variant of butterfly pea exhibited higher levels of anthocyanin content (138.842 mg/L) and total phenolic content (657.363 mg of GAE/gram extract). The addition of 2% L-Histidine effectively stabilized the anthocyanin compound. Autoclave sterilization demonstrated efficacy in ensuring extract sterility. In addition, the commercial products without herbal ingredients exhibited a larger inhibition zone of 12.3 mm, while the formulated herbal eye drops demonstrated significant inhibition of Staphylococcus aureus growth, with an inhibition zone diameter of 8.3 mm.